New paper:

July 8, 2015

Centre Affiliate, Dr Staffan Persson recently had a paper published in Nature Plants, titled “V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis

Below is a summary of the work discussed in the article:

The membrane that surrounds cells, also called the plasma membrane, is a defining feature for life, and activities that occur here are crucial for the cells to sense their environment and to protect themselves. Hence, proteins that can detect the cells surrounding and that can supply protective coats for the cells need to be delivered to the plasma membrane from within the cell. The protein delivering machinery in most cells consists of an elaborate system of membrane enclosed vesicles. These vesicles can be precisely targeted to the compartments for which they carry cargo. Proteins that are made inside of the cells can in this way be accurately delivered to the places where they are needed. In plant cells, indirect evidences have suggested that the pH of the vesicles that deliver proteins and other materials is important for the process to work effectively. Our work shows that the pH of the major vesicle station in the plant cell is regulated by a proton-ATPase. This ATPase transport protons into the vesicles and therefore makes them more acidic. The lower pH promotes vesicle delivery to the plasma membrane and to other compartments in the cell. We, furthermore, show that efficient delivery of an important hormone receptor and the enzyme that synthesise cellulose is dependent on the function of the ATPase. These results also explain the impaired growth characteristics of plants in which the ATPase was mutated. In summary, we have shown that maintenance of an acidic environment in vesicles that deliver two central components for plant growth is essential for normal plant growth